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Analysis of kelakai (stenochlaena palustris) and its antioxidative, lipase and lipid formation in adipocytes inhibitory capabilities

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With rising world obesity rates, weight management has become an emerging method to stay healthy. S. palustris which is rich in antioxidants, the lack of which may be related to obesity, was tested to see if it has any potential for managing obesity. Water, 50% methanol and methanol extracts of S. palustris were tested for lipase inhibitory activity and lipid accumulation inhibition in 3T3-L1 adipocytes. DPPH antioxidant activity was also tested. Water extract showed the highest lipase inhibition and methanol extract showed the highest lipid accumulation inhibition in adipocytes. Subsequent fractionation was done on the water extract into first water, 50% methanol and methanol fractions with DIAION HP20 gel. The 50% methanol and methanol fractions were fractionated again into water, 20%, 40%, 60%, 80% and methanol fractions with RP-18 gel. No relationship between antioxidant activity and lipase activity was found as the highest DPPH activity was found in the water fraction of the 50% methanol fraction and the highest lipase inhibitory activity in the methanol fraction of the methanol fraction. LC-MS data corroborate this conclusion.

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Availability

B03106

(Rack Thesis)

Available

Detail Information

Series Title

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Call Number

3106

Publisher

: Swiss German University., 2019

Collation

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Language

English

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Classification

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Content Type

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Media Type

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Carrier Type

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Edition

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Subject(s)

FT
Antioxidant
S. Palustris
Obesity
DPPH
Lipase
Adipocyte
Fractionation

Specific Detail Info

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