Ultrasonic pretreatment of seaweed and woodchips for the conversion of cellulose to glucose
In this study, cellulosic biomass from seaweed (Sargassum sp.) and lignocellulosic biomass from albacia woodchips (Paraserianthes falcataria) were given different pretreatment methods, ie. chemical (acid) and physical (ultrasonic), to convert cellulose to glucose for bioethanol production. 1% H2SO4 was applied for the acid pretreatment at 121oC. Ultrasonic pretreatment was carried out at varied time (10, 20 and 30 minutes) at 600 W, 20 kHz at ambient temperature. Additionally, enzymatic attack of the pretreated sample was also applied for the final hydrolysis of cellulose. The objective of the research was to determine the most effective ultrasonic duration, and the best combination of pretreatment method. The data showed that the highest glucose yield was achieved at 20 minutes ultrasonic time. Additionally, it was found that substantial amount of glucose was released during the ultrasonic stage. It is likely that ultrasonic treatment could promote hydrolysis of cellulose. The hydrolysis and pretreatment methods under optimum condition for seaweed produced 95.4 ┬▒ 9.9 mg glucose/g substrate and for woodchips was 84.0 ┬▒ 5.1 mg glucose/g substrate. Given that the conversion of glucose to bioethanol is 90%, thus the theoretical bioethanol yield would be 4.3% v/w for seaweed and 3.8% v/w for woodchips. In other words, one ton of seaweed and woodchips would produce 55 L and 48 L bioethanol, respectively.
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