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Spray drying of microbial kerantinase and study on its stability

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This research was aimed to prepare powder keratinase enzyme by spray drying method, to study the most suitable type and concentration of filler used in spray drying process, and to study the stability of spray-dried keratinase during storage. Microbial keratinase in this research was produced by keratinolytic bacteria (Brevibacillus thermoruber LII) that was isolated in a hot spring at Padang Cermin, Lampung using protocol developed by Zilda et al. (2012). Two types of filler were used in different concentration. They were tapioca and maltodextrin in 15%, 20% and 25% concentration. Meanwhile for the stability test were conducted in two different temperatures (room temperature and 4┬║ C). From the research, it was resulted that tapioca starch as a filler material with 15% concentration was able to protect keratinase obtaining the highest specific activity. However the product did not have good stability during storage. The most stable product was found in the enzyme that was encapsulated with 15% w/v maltodextrin, stored at 4o C for six weeks storage time.

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Availability

B01480

(wh)

Available

Detail Information

Series Title

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Call Number

1480

Publisher

: Swiss German University., 2013

Collation

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Language

English

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Classification

NONE

Content Type

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Carrier Type

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Edition

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Subject(s)

FT

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