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Prevalence of cervical cancer in endemic area (red light district) of Bandung and their characterization of isolated Human Papilloma Virus (HPV) from infected human

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Cervical cancer is one type of cancer that becomes the leading causes of deaths among woman in most countries. Almost all cervical cancers are caused by specific high risk type of Human Papilloma Virus (HPV) particularly type 16 and 18 that cause about 70% of the cases. In this research, the prevalence of cervical cancer in red light district of Bandung was determined and the nucleotide sequence of the isolate was analyzed to identify the type of HPV. The methodology used including Pap smear sample collection, cells staining, DNA extraction, Polymerase Chain Reaction (PCR), gel electrophoresis and nucleotide sequencing. The study concluded that the incidence of cervical cancer in Bandung area is 0% (2 ASC-US, 1 AG-US, 3 LSIL, 4 ASC-H, 5 HSIL and 0 cervical cancer). However, the prevalence of HPV infection in this area is 1.03% with one positive sample out of 97 screened women that is identified as HPV type 61 (using MY09/MY11 primer set) and considered as low risk type.

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B00840

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Detail Information

Series Title

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Call Number

840

Publisher

: Swiss German University., 2010

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Language

English

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Classification

NONE

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Subject(s)

BM

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