Stability improvement of anthocyanins from butterfly pea (clitoria ternatea l.) Flower extract with addition of surfactant
The effects of anionic, cationic and non-ionic surfactants on the stability of anthocyanin from Clitoria ternatea flower anthocyanin extract (CTAE) at pH 7 were studied using UV spectrophotometer analysis. The interaction of CTAE with anionic surfactants, sodium dodecyl sulfate (SDS) and sodium dodecylbenzene sulfonate (SDBS) and a cationic surfactant, dodecyltrimethylammonium bromide (DTAB) were able to prolong the stability of CTAE. SDS and SDBS seemed to preferentially stabilize the red cationic form AH+ while DTAB towards the blue anionic A-. Strong electrostatic attraction appeared to be necessary to attain the highest stability. On the other hand, cetyltrimethylammonium bromide (CTAB), also a cationic surfactant, demonstrated a contradictory effect which might be caused by electrostatic repulsion due to its higher hydrophobicity. Stability of CTAE was found to not change in the presence of non-ionic surfactants. Stability improvement of CTAE in SDS led to further examination of its effects in various concentration (0.25%, 0.5% and 1%) and at pH 4 to 7. Positive effect was observed at pH 6 to 7 upon increasing concentration as evidenced in a shorter hypsochromic shift and delayed color loss of CTAE, substantially at 1% SDS. Yet, SDS appeared to be ineffective at pH 4 to 5 at all concentrations. Collectively, stabilizationrnof CTAE was dependent to the whole chemical structure of surfactant, not only to the charge of the head groups.
B03104 | (Rack Thesis) | Available |
No other version available