Development of mesenchymal stem cells (mscs) from dental pulp of macaca fascicularis
The dental pulp of Macaca fascicularis was isolated and cultured to developrnMesenchymal Stem Cells (MSCs). The morphology of fibroblast-like cells andrnprotein surface markers, including CD44, CD73, CD90, and CD105 were used torndetermine the characteristics of MSCs. Markers tests were conducted using RT-PCR.rnAfterwards, the dental pulp stem cells (DPSCs) were cultured in certainrndifferentiation media to differentiate them into osteocytes, chondrocytes, andrnadipocytes. The research showed that morphologically all of those differentiationsrnwere formed.rnIn addition, hematopoietic characteristics were also tested by CD34 and CD45 surfacernmarkers which were negatively expressed. Furthermore, pluripotent ability of stemrncells also analyzed by Nanog, Oct4, and Sox2 markers which were negativelyrnexpressed too. Ultimately, the DPSCs of Macaca fascicularis as a non-human primaternmight be used as a model of human diseases.
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