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Purification of -Amylase Enzyme Produced in Solid State Fermentation Using Sorghum Grain as Substrate

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Amylases are one of the most important and oldest industrial enzymes. Alpha amylase involve in energy production by breaking down starch into sugar that is used later to produce energy. Sorghum grain can be used as a substrate in the production of ?í¬±-amylase in Solid State Fermentation. in purification of ?í¬±-amylase in Solid State Fermentation using sorghum grain as substrate, multi-precipitation and drying technique was adopted. Every stages of precipitation at pH 5, pH 6, pH 8 and pH 9 were analysed to obtain the specific activity of enzyme using reducing sugar by DNS method and protein content determination. Multi-precipitation process of enzyme by pH shifting was capable to increase specific activity enzyme using the multi-precipitation process followed by drying in purification increased the specific activity of the enzyme by 41.5X U/mg.

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Availability

B01745

(Rack Thesis)

Available but not for loan - Missing

Detail Information

Series Title

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Call Number

1745

Publisher

: Swiss German University., 2014

Collation

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Language

English

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

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Carrier Type

-

Edition

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Subject(s)

PE
Solid-state fermentation
Sorghum grain
Multi-precipitation
Drying technique
Specific activity
?í¬±-amylase

Specific Detail Info

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