Sugarcane Bagasse Utilization as Solid State Fermentation (SSF) Substrate for ?-Amylase Production
This research investigated the ability of sugarcane bagasse as substrate in SSF to produce ?í¬±-amylase. the sugarcane bagasse was inoculated with Aspergillus oryzae, Saccharomyces cerevisiae, and the mixed of those pure cultures, in initial moisture content of 50% and 70%. After 72 hour fermentation, ?í¬±-amylase was extracted and purified partially. Using the DNS method and protein content analysis, it was determined that ?í¬±-amylase with the highest activity was that of Aspergillus oryzae with 70% of the initial moisture content. the research investigation was continued by combining sugar cane bagasse and rice bran with 5:0, 3:2, and 2:3 ratio to check the influence of substrate type of the enzyme production. the produced ?í¬±-amylase was analyzed at 24 hour interval. Sugarcane bagasse and rice bran of 5:0 ratio showed the highest enzyme and specific activity with 1.25 mg (ml. Min) -1 and 0.47 mg (ml. Min) -1 respectively at 48 hour fermentation.
B01760 | (Rack Thesis) | Available |
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