print

Production of ?- amylase in solid state fermentation by using sorghum grain as substrate

---

The usage of sorghum (Sorghum bicolor (L.) Moench) as solid state fermentation (SSF) substrate for ?-amylase enzyme production was studied in this research. Two factors were used for analysis, ratio between sorghum with rice bran and initial moisture content. Microorganism that showed amylolytic activities was isolated from ragi tapai. Five types of amylolytic microbes strains discovered were cultured in SSF with sorghum and compared with one and another to see its activity and analysed for finding strain with the most optimum amylolytic activities. From the analysis, it was found that, the ratio of sorghum with rice bran along with the yeast strains give no significant impact to the microbes growth rate. Initial moisture content was proportional with microbial growth. The activity of ?-amylase enzyme produced was analysed using reducing sugar method. SSF with isolated strain number 5 exhibited the highest ?-amylase activity 6.16x10-3 /minutes. Based on those facts, it can be concluded that sorghum grain is suitable to be used for producing ?-amylase enzyme using ragi tapai.

---

Availability

B01484

(wh)

Available

Detail Information

Series Title

-

Call Number

1484

Publisher

: Swiss German University., 2013

Collation

-

Language

English

ISBN/ISSN

-

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

-

Subject(s)

FT

Specific Detail Info

-

Statement of Responsibility

-

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment