Sugarcane Bagasse Utilization as Solid State Fermentation (SSF) Substrate for ?-Amylase Production

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Sugarcane Bagasse Utilization as Solid State Fermentation (SSF) Substrate for ?-Amylase Production

Hardi, Inez Areta - Personal Name; Prabawati, Elisabeth K. - Personal Name; Kartawiria, Irvan S. - Personal Name;

This research investigated the ability of sugarcane bagasse as substrate in SSF to produce ?├¡┬¼┬▒-amylase. the sugarcane bagasse was inoculated with Aspergillus oryzae, Saccharomyces cerevisiae, and the mixed of those pure cultures, in initial moisture content of 50% and 70%. After 72 hour fermentation, ?├¡┬¼┬▒-amylase was extracted and purified partially. Using the DNS method and protein content analysis, it was determined that ?├¡┬¼┬▒-amylase with the highest activity was that of Aspergillus oryzae with 70% of the initial moisture content. the research investigation was continued by combining sugar cane bagasse and rice bran with 5:0, 3:2, and 2:3 ratio to check the influence of substrate type of the enzyme production. the produced ?├¡┬¼┬▒-amylase was analyzed at 24 hour interval. Sugarcane bagasse and rice bran of 5:0 ratio showed the highest enzyme and specific activity with 1.25 mg (ml. Min) -1 and 0.47 mg (ml. Min) -1 respectively at 48 hour fermentation.

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B01760 (Rack Thesis) Available

Detail Information

Series Title: -
Call Number: 1760
Publisher: : Swiss German University., 2014
Collation: -
Language: English
ISBN/ISSN: -
Classification: NONE
Content Type: -
Media Type: -
Carrier Type: -
Edition: -
Subject(s): FT
activity
?í¬±-amylase
Sugarcane bagasse
Solid state fermentation
A. oryzae
Specific Detail Info: -
Statement of Responsibility: -

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